Add README.md

README.md

+# **VTA snRNA-seq**
+
+
+This page contains R code for analysis of single-nucleus RNA-seq (snRNA-seq) datasets from "An Atlas of Transcriptionally Distinct Cell Populations within the Rat Ventral Tegmental Area".  
+
+
+## **Study design**
+
+This snRNA-seq dataset was generated from VTA punches from naive adult male and female Sprague Dawley rats.  
+
+Datasets in this preprint used the 10X Genomics Chromium Single Cell 3’ library construction kit to generate barcoded libraries for Illumina sequencing. For each dataset, we provide R Markdown and Word documents containing analysis parameters and code.  
+
+
+## **Citation**
+
+Phillips III, R.A.* Tuscher, J.J.*, Black, S.L., Ianov, L., & Day, J.J. (2021). A transcriptional atlas of the rat ventral tegmental area at single cell resolution. *bioRxiv* DOI:
+[Link]()
+
+
+## **Links**
+
+All Day lab resources may be found at the [Day Lab website](http://day-lab.org/resources)  
+[Ratlas Shiny App](https://day-lab.shinyapps.io/ratlas/)  
+[BioRxiv preprint]()  
+
+
+## **Raw data**
+
+GEO accession [GSE137763]()  
+
+
+## **Source code**
+All packages required for reproduction of this analysis are included in the session info section of the word doc included in this repository.
+[Seurat v3.2.2](https://github.com/satijalab/seurat)